1.- PUBLICATIONS IN SCI CITATION INDEX
OLMOS, A., DASÍ, M.A., CANDRESSE, T., CAMBRA, M. 1996.
Print capture PCR: a simple and highly sensitive method for
the detection of plum pox virus (PPV) in plant
tissues. Nucleic Acids Research 24,
2192-2193.
OLMOS, A., CAMBRA, M., DASÍ, M.A., CANDRESSE, T., ESTEBAN,
O., GORRIS, M.T., ASENSIO, M., 1997. Simultaneous detection
and typing of plum pox potyvirus (PPV) isolates by
heminested-PCR and PCR-ELISA. Journal of Virological Methods 68,
127-137.
OLMOS, A., CAMARENA, J.J., NOGUEIRA, J.M., NAVARRO, J.C.,
RISEN, J., SANCHEZ, R., 1998. Application of an optimized
and highly discriminative method based on arbitrarily
primed PCR for epidemiologic analysis of
methicillin-resistant Staphylococcus aureus nosocomial
infections. Journal of Clinical Microbiology 36,
1128-1134.
CAMARENA, J.J., ARTERO, A., NOGUEIRA, J.M., NAVARRO, J.C.,
OLMOS, A., FRANCO, J., BLANQUER, R., 1998. Tuberculosis en
pacientes con SIDA: Aportación del análisis mediante
polimorfismos de los fragmentos de restricción de aislados
de Mycobacterium tuberculosis. Medicina clínica (Barcelona)
111, 721-724.
CANDRESSE, T., CAMBRA, M., DALLOT, S., LANNEAU, N.,
ASENSIO, M., GORRIS, M.T., REVERS, F., MACQUAIRE, G.,
OLMOS, A., BOSCIA, D., QUIOT, J.B., DUNEZ, J., 1998.
Comparison of monoclonal antibodies and polymerase chain
reaction assays for the typing of isolates belonging to the
D and M serotypes of plum pox potyvirus.
Phytopathology 88,
198-204.
OLMOS, A., CAMBRA, M., ESTEBAN, O., GORRIS, M.T., TERRADA,
E., 1999. New device and method for capture, reverse
transcription and nested PCR in a single closed
tube. Nucleic Acids Research 27,
1564-1565.
NOGUEIRA, J.M., FRANCO, J., CAMARENA, J.J., NAVARRO, J.C.,
OLMOS, A., BLANQUER, R., ARTERO, A., 2000. Molecular
approach to identifying route of Mycobacterium tuberculosis
transmission in a village. International Journal of
Tuberculosis Lung Disease 4, Lett., 91-92.
CAMBRA, M., GORRIS, M.T., MARROQUÍN, C., ROMÁN, M.P.,
OLMOS, A., MARTÍNEZ, M.C., HERMOSO DE MENDOZA, A., LÓPEZ,
A,. NAVARRO, L., 2000. Incidence and epidemiology of Citrus
tristeza virus in the Valencian Community of Spain. Virus
Research 71,75-85.
SAMBADE, A., MARTÍN, S., OLMOS, A., GARCÍA, M.L., CAMBRA,
M., GRAU, O., GUERRI, J., MORENO, P., 2000. A fast one-step
reverse transcription and polymerase chain reaction
(RT-PCR) amplification procedure providing highly specific
complementary DNA from plant virus RNA. Journal of
Virological Methods 87, 25-28.
BERTOLINI, E., OLMOS, A., MARTÍNEZ, M.C., GORRIS, M.T.,
CAMBRA, M., 2001. Single-step multiplex RT-PCR for
simultaneous and colourimetric detection of six RNA viruses
in olive trees. Journal of Virological Methods 96, 33-41.
GROTE, D., OLMOS, A., KOFOET, A., TUSET, J.J., BERTOLINI,
E., CAMBRA, M. 2002. Specific and sensitive detection of
Phytophthora nicotianae by simple and nested-PCR.
European Journal of Plant Pathology 108,
197-207.
OLMOS, A., BERTOLINI, E., CAMBRA, M. 2002. Simultaneous and
Co-operational amplification (Co-PCR) for detection of
plant viruses. Journal of Virological Methods 106,51-59.
BERTOLINI, E., PEÑALVER, R., GARCÍA, A., OLMOS, A.,
QUESADA, J. M.; CAMBRA, M., LÓPEZ, M. M. 2003. Highly
sensitive detection of Pseudomonas savastanoi pv.
savastanoi in asymptomatic olive plants by nested-PCR in a
single closed tube. Journal of Microbiological Methods
52,261-266.
BERTOLINI, E., OLMOS, A, LÓPEZ, M.M., CAMBRA, M. 2003.
Multiplex nested RT-PCR in a single cloed tube for
sensitive and simultaneous detection of four RNA viruses
and Pseudomonas savastanoi pv. savastanoi in olive trees.
Phytopathology 93,286-292.
TORRES, P., CAMARENA, J.J., GÓMEZ, J.R., NOGUEIRA, J.M.,
GIMENO, V., NAVARRO, J.C., OLMOS, A. 2003. Comparison of
PCR mediated amplification of DNA and the classical methods
for detection of Mycobacterium leprae in different types of
clinical samples in leprosy patients and contacts. Leprosy
review 74, 18-30.
MARROQUIN, C., OLMOS, A., GORRIS, M.T., BERTOLINI, E.,
MARTÍNEZ, M.C., CARBONELL, E., HERMOSO DE MENDOZA, A.,
CAMBRA, M. 2004. Estimation of the number of aphids
carrying Citrus tristeza virus, that visit adult citrus
trees. Virus Research 100,
101-108.
OLMOS, A., BERTOLINI, E., GIL, M., CAMBRA, M. 2005.
Real-time assay for quantitative detection of
nonpersistently transmitted Plum pox virus RNA targets in
single aphids. Journal of Virological Methods 128,
151-155.
CAPOTE, N., GORRIS, M.T., MARTÍNEZ, M.C., ASENSIO, M.,
OLMOS, A., CAMBRA, M. 2006. Interference between D and M
types of Plum pox virus in Japanese plum assessed by
specific monoclonal antibodies and quantitative real-time
reverse transcription-polymerase chain reaction.
Phytopathology 96, 320-325.
OLMOS., A., BERTOLINI, E., CAMBRA, M. 2007. Isothermal
amplification coupled with rapid flow-through hybridisation
for sensitive diagnosis of Plum pox virus.
Journal of Virological Methods 139,
111-115.
BERTOLINI, E., TORRES, E., OLMOS, A., MARTÍN, M.P.,
BERTACCINI, A., CAMBRA, M. 2007. Co-operational PCR coupled
with dot-blot hybridization for detection and 16SrX
grouping of phytoplasmas. Plant Pathology 56,
677-682.
MORENO, A., BERTOLINI, E., OLMOS, A., CAMBRA, M., FERERES,
A. 2007. Estimation of vector propensity for Lettuce mosaic
virus based on viral detection in single aphids. Spanish
Journal of Agricultural Research 5, 376-384.
BERTOLINI,
E., MORENO, A., CAPOTE, N., OLMOS, A., DE LUIS, A., VIDAL,
E., PEREZ-PANADES, J., CAMBRA, M. 2008. Quantitative
detection of Citrus tristeza virus in plant tissues and
single aphids by real-time RT-PCR. European Journal of
Plant Pathology 120, 177-188.
MASSART, S., BROSTAUX, Y.,
BARBAROSSA, L., CÉSAR, V., CIESLINSKA, M., DUTRECQ, O.,
FONSECA, F., GUILLEM, R., LAVIÑA, A., OLMOS, A., STEYER,
S., WETZEL, T., KUMMERT, J., JIJAKLI, M.H. 2008. Inter-laboratory evaluation of a duplex
RT-PCR method using crude extracts for the simultaneous
detection of Prune dwarf virus and Prunus necrotic ringspot
virus. European Journal of Plant Pathology (In
press).
2.-
OTHER SCIENTIFIC PUBLICATIONS
CAMBRA, M., OLMOS, A., CANDRESSE, T., GORRIS, M.T., 1998.
Detection of prunus viruses by print-capture PCR. Bulletin
OEPP/EPPO Bulletin 27, 535-538.
GROTE, D., OLMOS, A., KOFOET, A., TUSET, J.J., BERTOLINI,
E., CAMBRA, M., 1998. Detection of Phytophthora nicotianae
by PCR. Bulletin OEPP/EPPO Bulletin 30, 539-542.
LÓPEZ, M.M., BERTOLINI, E., OLMOS, A., CARUSO, P., GORRIS,
M.T., LLOP, P., PENYALVER, R., CAMBRA, M. 2003. Innovative
tools for detection of plant pathogenic viruses and
bacteria. International Microbiology 6, 233-243.
OLMOS. A., CAPOTE, N., CANDRESSE, T., 2006. Molecular
detection and characterisation of Plum pox virus. Bulletin
OEPP/EPPO Bulletin 36, 262-266.
OLMOS. A., BERTOLINI, E., CAPOTE, N., CAMBRA,
M. 2008. An Evidence-Based Approach to Plum Pox Virus
Detection by DASI-ELISA and RT-PCR in Dormant Period.
Virology: Research and Treatment 1, 1- 8.
3.-
BOOK CHAPTERS.
CAMBRA,
M., OLMOS, A., GORRIS, M.T., DURAN, N., ROMÁN, M.P.,
CAMARASA, E., DASÍ, M.A. (1997). Sensitive detection of
plant pathogens by using immobilized targets in tissue
imprinted membranes. 95-98. In: Diagnosis and
identification of plant pathogens. Eds: H.W. Dehne, G.
Adam, M. Diekmann, J. Frahm, A. Mauler-Machnik and P. van
Halteren. Kluwer Academic Pusblishers, London, 556 pp.
(ISBN: 0-7923-4771-4).
PENYALVER,
R., BERTOLINI, E., OLMOS, A., GARCÍA, A., CAMBRA, M.,
LÓPEZ, M.M., 2000. Detection of P.savastanoi pv. savastanoi
on asymptomatic olive plant tissues by enrichment-PCR.
421-424. In: Plant Pathogenic Bacteria. Ed. S. H. De Boer.
Kluwer Academic Publishers, Dordretch, 545 pp.
(ISBN:0-7923-7110-0).
OLMOS,
A., ESTEBAN, O., BERTOLINI, E., CAMBRA, M. (2003). Nested
RT-PCR in a single closed tube. 153-161. In: Methods in
Molecular Biology vol. 226. PCR Protocols. Second Edition.
Bartlett, J.M.S., Stirling, D. (Eds.). Humana Press.Totowa,
USA. 500 pp. (ISBN: 0-89603-642-1).
BERTOLINI,
E., CARUSO, P., PENYALVER, R., OLMOS, A., QUESADA, J.M.,
CAMBRA, M., LÓPEZ, M.M. (2003). Optimising PCR detection of
Ralstonia solanacearum and Pseudomonas savastanoi pv.
savastanoi: two models, to approaches. 523-529. In:
Pseudomonas syringae and related pathogens. Eds. N.S.
Iacobellis et al., Kluwer Academic Publishers, Dordretch,
724 pp. (ISBN:1-4020-1227-6).
CAMBRA,
M., BERTOLINI, E., OLMOS, A., CAPOTE, N. (2005). Molecular
methods for detection and quantitation of virus in aphids.
In: KUEHNE, Thomas. (Org). Significance of Virus disease
for crop biosecurity in a developing European Community.
Dodretch.
OLMOS, A., CAPOTE, N., BERTOLINI, E., CAMBRA, M. (2007).
Molecular diagnostic methods for plant viruses. In: Punja,
Z.K., De Boer, S., Sanfancon, H.I. (eds.). Biotechnology
and plant disease management. CABI Publishing, pp. 227-249.
4.-
PATENTS
Títle: Procedimiento para la preparación de dianas para la
reacción en cadena de la polimerasa (PCR).
Número de solicitud: 9601155
Fecha de presentación: 24.05.1996
Fecha de publicación de la solicitud: 16.02.1998
Fecha de concesión: 04.07.1998
Fecha de anuncio de la concesión: 01.10.1998
Fecha de publicación: 01.10.1998
Títle: Dispositivo compartimentado y método para la captura
y doble amplificación enzimática de secuencias diana de
ácidos nucleicos en un solo dispositivo compartimentado
cerrado.
Número de solicitud: 9801642
Fecha de presentación: 31.07.1998
Fecha de publicación de la solicitud: 01.03.2001
Fecha de concesión: 08.08.2001
Fecha de anuncio de la concesión: 16.11.2001
Fecha de publicación: 16.11.2001
Títle: Método y mezcla de reacción para la amplificación
enzimática simultánea, coordinada y cooperativa de
secuencias diana de ácidos nucleicos.
Número de solicitud: 200002613
Fecha de presentación: 31.10.2000
Fecha de publicación de la solicitud: 16.03.2003
Fecha de concesión: 16.01.2003
Fecha de anuncio de la concesión: 16.02.2004
Fecha de publicación: 16.02.2004
